Ph-ncas9-ppe
http://www.plantgenomeediting.net/ WebbpH-nCas9-PBE.gb A portion of this plasmid was derived from a plasmid made by APOBEC1, XTEN, nCas9 (D10A), and UGI sequences were codon-optimized for cereal …
Ph-ncas9-ppe
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WebbpH-nCas9-PBE质粒,产品图例: 一、扩增流程 收到产品后,请先根据产品管壁标签来判断产品形式,并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。 1、质粒干粉(常温运输,存于-20度,90天保质期,请务必转化挑单克隆培养,不要直接使用和测序) ①收到质粒干粉后请先5000rpm离心1min,再加入20μl ddH2O去离子水溶解质粒; ②取1 … WebbpH-nCas9-PPE-V2 pSpG-PPE pH-ePPE ePPE pRN120 Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn …
WebbpOsU3 pTaU3 pH-nCas9-PPE-V2/pH-ePPE Forward primer (5'-3'): + Spacer sequence + Reverse primer (5'-3'): + PBS and RT template sequence + Submit >> clear. Help document. There are 35458 Visits, latest from 52.167.144.144 ... WebbThe pOsU3, pTaU3, and pTaU6 vectors are recommended for particle bombardment and protoplast transformation experiments. The pH-nCas9-PPE-V2 vector is recommended …
WebbpH-nCas9-PPE-V2 Plasmid #170131 Purpose For plant prime editing in rice plants or monocotyledons protoplasts Depositor Caixia Gao Article Lin et al Nat Biotechnol. 2024 Mar 25. pii: 10.103 Insert nCas9 (H840A)-M-MLV Use CRISPR Tags Expression Plant Mutation H840A for Cas9; D200N, T306K, W313F, T330P and L… Promoter maize … Webb27 feb. 2024 · Examination of 92 pH-nCas9-PBE-transformed plants revealed that 40 harbored at least one C to T substitution in the target region (mutation efficiency of 43.48%) ( Fig. 2d and Supplementary...
Webb21 feb. 2024 · Cas9 protein (1 μg) and sgRNA (1 μg) were mixed with the purified target DNA (100–150 ng) in Cas9 reaction buffer (20 mM HEPES, pH 7.5, 150 mM KCl, 10 mM MgCl 2, 0.5 mM DTT) in a total volume of 20 μl, followed by digestion at 37 °C for 0.5–1 h.
WebbPlasmid pH-nCas9-PPE-V2 from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 Mar 25. pii: 10.1038/s41587-021-00868-w. … inbox emails chromeWebbLocation : Home > Faculty > Caixia Gao > Selected Publications. Selected Publications. Publications (* Corresponding author; 1 co-first author) 1. Wang S1, Zong Y1, Lin Q1, Zhang H1, Chai Z, Zhang D, Chen K, Qiu JL, Gao C * (2024) Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC-Cas9. in animals regulation is carried out by theinbox empty outlookWebbLin Q, Zong Y, Xue C, Wang S, Jin S, Zhu Z, et al.Prime genome editing in rice and wheat. Nat Biotechnol. 2024;38:582-585 pubmed publisher in animals oxygenWebb16 mars 2024 · The pH-nCas9-PPE3 binary vectors were transformed into Agrobacterium tumefaciens strain EHA105 by electroporation. Callus cells of Zhonghua11 were … inbox emailwebaccess.co.ukWebbPlasmid pH-nCas9-PPE from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 May;38(5):582-585. doi: 10.1038/s41587-020 … inbox emails of pawittar singh bhandarihttp://www.biofeng.com/adjy/zhili/pH-nCas9-PBE.html inbox emails disappeared outlook 365