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WebDec 10, 2024 · The reconstructed pGL3-Promoter was introduced into the MC3T3-E1 pre-osteoblasts along with the pRL-TK-Renilla reporter (Promega Corporation) using Lipofectamine ® 3000 reagent (Thermo Fisher Scientific, Inc.) following manufacturer's protocol for 4-6 h at 37˚C. Following 6 h of transfection, cells were treated with the miR … WebMar 13, 2024 · The anti-glucagon antibody (2760S) was from CST Biological Reagents Company Limited (Shanghai, China). The anti-mast cell function-associated antigen B (MAFB) antibody (DF8895) and anti-PDX1 antibody (DF7170) were from Affinity Biosciences (Cincinnati, OH, USA). The anti-neurogenin 3 (NGN3) antibody (GTX60254) was from … somers pointe country club
Cell Signaling Technology (CST): Antibodies, Reagents, …
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